INCREASED AGE AND BODY MASS INDEX RELATES TO INCREASED TIME-LAPSE VARIABILITY AMONG EMBRYOS FROM THE SAME CYCLE

The negative effect of the increased age and obesity on women’s fertility, natural conception assisted reproduction is already well known. To date, it still not fully clear how these important factors could influence different aspects embryo development morphokinetics. aim present study was to assess association between inter-embryo variability morphokinetic parameters patient body mass index (BMI). A total 305 women (967 embryos) were included for analysis in this study. All embryos cultured a single culture medium (Life Global, Brussels, Belgium) time-lapse system (EmbryoScope, Vitrolife, Sweden). Only with more than 2 per cycle Fifteen analyzed: time pronuclei appearance (tPNa), fading (tPNf), cleavage times (t2, t3, t4, t5, t6, t7, t8, t9), morulae formation (tM), starting blastulation (tSB), full blastocyst stage (tB), expansion (tEB) hatching timing (tHB). Statistical performed using IBM SPSS Software v.21. Inter-embryo each woman/cycle measured as coefficient variation (CV). Spearman correlation done find coefficients variables female BMI. Women showed low but significant positive (CV) certain – t7 (r=0.17, p=0.006), t8 (r=0.22, t9 (r=0.13, p=0.048), tB (r=0.18, p=0.022). Moreover, BMI also relatively higher number CV variables, including earlier events t3 (r=0.20, p=0.038), t4 (r=0.25, p=0.012), t5 (r=0.24, p=0.02), t6 (r=0.26, (r=0.23, p=0.03) p=0.045). In conclusion, human associated Embryo morphokinetics differs initial stages according later age.